Overexpression of mtlD gene in transgenic Populus tomentosa improves salt tolerance through accumulation of mannitol.
Identifieur interne : 003F83 ( Main/Exploration ); précédent : 003F82; suivant : 003F84Overexpression of mtlD gene in transgenic Populus tomentosa improves salt tolerance through accumulation of mannitol.
Auteurs : Lei Hu [République populaire de Chine] ; Hai Lu ; Qunlu Liu ; Xuemei Chen ; Xiangning JiangSource :
- Tree physiology [ 0829-318X ] ; 2005.
Descripteurs français
- KwdFr :
- Arbres (génétique), Arbres (physiologie), Chlorure de sodium (métabolisme), Escherichia coli (génétique), Gènes bactériens (génétique), Mannitol (métabolisme), Populus (génétique), Populus (physiologie), Sugar alcohol dehydrogenases (génétique), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (physiologie).
- MESH :
- génétique : Arbres, Escherichia coli, Gènes bactériens, Populus, Sugar alcohol dehydrogenases, Végétaux génétiquement modifiés.
- métabolisme : Chlorure de sodium, Mannitol.
- physiologie : Arbres, Populus, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Escherichia coli (genetics), Genes, Bacterial (genetics), Mannitol (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (physiology), Populus (genetics), Populus (physiology), Sodium Chloride (metabolism), Sugar Alcohol Dehydrogenases (genetics), Trees (genetics), Trees (physiology).
- MESH :
- chemical , genetics : Sugar Alcohol Dehydrogenases.
- chemical , metabolism : Mannitol, Sodium Chloride.
- genetics : Escherichia coli, Genes, Bacterial, Plants, Genetically Modified, Populus, Trees.
- physiology : Plants, Genetically Modified, Populus, Trees.
Abstract
The mtlD gene encoding mannitol-1-phosphate dehydrogenase, which catalyzes the biosynthesis of mannitol from fructose, was cloned from Escherichia coli and transferred to poplar (Populus tomentosa Carr.) through Agrobacterium-mediated transformation. The transgenic plants were screened and selected on Murashige and Skoog (MS) medium containing 30-50 mg l(-1) kanamycin and verified by polymerase chain reaction (PCR) and Southern blotting. Expression of the gene led to synthesis and accumulation of mannitol in the transgenic plants. Gas chromatography and mass spectrometry (GC/MS) and capillary gas chromatography (GC) showed that transgenic plants accumulated much more mannitol in their tissues than the wild-type plants, whether cultured in vitro, or grown hydroponically or in the field. Increased salt tolerance of transgenic plants was observed both in vitro and in hydroponic culture. The transgenic buds rooted normally on MS medium containing 50 mM NaCl, whereas wild-type buds did not. In the 40-day hydroponic experiments, transgenic poplar plants survived in a 75-mM NaCl treatment, whereas the wild-type poplar plants tolerated only 25 mM NaCl. Under the same NaCl stress, stomatal conductance, transpiration rates and photosynthetic rates were all higher in transgenic plants than in wild-type plants, whereas cellular relative conductivity was lower. We demonstrated that the mtlD gene was expressed in transgenic poplar plants, resulting either directly or indirectly in mannitol accumulation and improved salt tolerance. The constant mannitol concentrations in transgenic plants during the NaCl treatments indicated that mannitol accumulation caused by the mtlD gene was not a consequence of NaCl stress. Height growth was reduced by about 50% in the transgenic plants compared with the wild-type plants in the absence of salt; however, relative growth rate was much less influenced by salt stress in transgenic plants than in wild-type plants. The stunted growth of the transgenic plants may in part explain their improved salt tolerance.
DOI: 10.1093/treephys/25.10.1273
PubMed: 16076776
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Lu, Hai" sort="Lu, Hai" uniqKey="Lu H" first="Hai" last="Lu">Hai Lu</name>
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<author><name sortKey="Chen, Xuemei" sort="Chen, Xuemei" uniqKey="Chen X" first="Xuemei" last="Chen">Xuemei Chen</name>
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<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (physiology)</term>
<term>Populus (genetics)</term>
<term>Populus (physiology)</term>
<term>Sodium Chloride (metabolism)</term>
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<term>Arbres (physiologie)</term>
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<term>Gènes bactériens (génétique)</term>
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<front><div type="abstract" xml:lang="en">The mtlD gene encoding mannitol-1-phosphate dehydrogenase, which catalyzes the biosynthesis of mannitol from fructose, was cloned from Escherichia coli and transferred to poplar (Populus tomentosa Carr.) through Agrobacterium-mediated transformation. The transgenic plants were screened and selected on Murashige and Skoog (MS) medium containing 30-50 mg l(-1) kanamycin and verified by polymerase chain reaction (PCR) and Southern blotting. Expression of the gene led to synthesis and accumulation of mannitol in the transgenic plants. Gas chromatography and mass spectrometry (GC/MS) and capillary gas chromatography (GC) showed that transgenic plants accumulated much more mannitol in their tissues than the wild-type plants, whether cultured in vitro, or grown hydroponically or in the field. Increased salt tolerance of transgenic plants was observed both in vitro and in hydroponic culture. The transgenic buds rooted normally on MS medium containing 50 mM NaCl, whereas wild-type buds did not. In the 40-day hydroponic experiments, transgenic poplar plants survived in a 75-mM NaCl treatment, whereas the wild-type poplar plants tolerated only 25 mM NaCl. Under the same NaCl stress, stomatal conductance, transpiration rates and photosynthetic rates were all higher in transgenic plants than in wild-type plants, whereas cellular relative conductivity was lower. We demonstrated that the mtlD gene was expressed in transgenic poplar plants, resulting either directly or indirectly in mannitol accumulation and improved salt tolerance. The constant mannitol concentrations in transgenic plants during the NaCl treatments indicated that mannitol accumulation caused by the mtlD gene was not a consequence of NaCl stress. Height growth was reduced by about 50% in the transgenic plants compared with the wild-type plants in the absence of salt; however, relative growth rate was much less influenced by salt stress in transgenic plants than in wild-type plants. The stunted growth of the transgenic plants may in part explain their improved salt tolerance.</div>
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<Abstract><AbstractText>The mtlD gene encoding mannitol-1-phosphate dehydrogenase, which catalyzes the biosynthesis of mannitol from fructose, was cloned from Escherichia coli and transferred to poplar (Populus tomentosa Carr.) through Agrobacterium-mediated transformation. The transgenic plants were screened and selected on Murashige and Skoog (MS) medium containing 30-50 mg l(-1) kanamycin and verified by polymerase chain reaction (PCR) and Southern blotting. Expression of the gene led to synthesis and accumulation of mannitol in the transgenic plants. Gas chromatography and mass spectrometry (GC/MS) and capillary gas chromatography (GC) showed that transgenic plants accumulated much more mannitol in their tissues than the wild-type plants, whether cultured in vitro, or grown hydroponically or in the field. Increased salt tolerance of transgenic plants was observed both in vitro and in hydroponic culture. The transgenic buds rooted normally on MS medium containing 50 mM NaCl, whereas wild-type buds did not. In the 40-day hydroponic experiments, transgenic poplar plants survived in a 75-mM NaCl treatment, whereas the wild-type poplar plants tolerated only 25 mM NaCl. Under the same NaCl stress, stomatal conductance, transpiration rates and photosynthetic rates were all higher in transgenic plants than in wild-type plants, whereas cellular relative conductivity was lower. We demonstrated that the mtlD gene was expressed in transgenic poplar plants, resulting either directly or indirectly in mannitol accumulation and improved salt tolerance. The constant mannitol concentrations in transgenic plants during the NaCl treatments indicated that mannitol accumulation caused by the mtlD gene was not a consequence of NaCl stress. Height growth was reduced by about 50% in the transgenic plants compared with the wild-type plants in the absence of salt; however, relative growth rate was much less influenced by salt stress in transgenic plants than in wild-type plants. The stunted growth of the transgenic plants may in part explain their improved salt tolerance.</AbstractText>
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